Review

fixation buffer (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher fixation buffer
Fixation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+buffer/HEPES/pm42001993-277-4-62
Average 99 stars, based on 1 article reviews

fixation buffer - by Bioz Stars, 2026-11

99/100 stars

Images

Incubation:

Article Title: STAT3 inhibition in combination with CD47 blockade inhibits osteosarcoma lung metastasis.
Article Snippet: .. After incubation, cells were washed in FACS buffer and fixed using fixation buffer (Thermofisher). .. For intracellular/intranuclear staining, fixed cells were washed with permeabilization buffer followed by staining with antibody in the buffer for 30 mins at 4 C. Cells were acquired using BD Fortessa and analyzed using FlowJo v. 10 software. frontiersin.org

Article Title: A fluorescent sensor for real-time monitoring of DPP8/9 reveals crucial roles in immunity and cancer
Article Snippet: .. After the treatment/induction period, the media were removed, and cells were washed with 1 ml PBS and incubated with 1 ml fixation buffer (4% PFA in PBS; Thermo Fisher Scientific) for 15 min at RT. .. Then, cells were washed 3x with 1 ml PBS and incubated with 0.5 ml of 4′,6-diamidino-2-phenylindole (DAPI) solution (1 μg/ml; Roth) for 15 min at RT.

Article Title: A fluorescent sensor for real-time monitoring of DPP8/9 reveals crucial roles in immunity and cancer.
Article Snippet: .. After the treatment/induction period, the media were removed, and cells were washed with 1 ml PBS and incubated with 1 ml fixation buffer (4% PFA in PBS; Thermo Fisher Scientific) for 15 min at RT. .. Then, cells were washed 3x with 1 ml PBS and incubated with 0.5 ml of 49,6-diamidino-2phenylindole (DAPI) solution (1 μg/ml; Roth) for 15 min at RT.

Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
Article Snippet: .. After incubation for 20 min on ice, cells were washed once with FACS buffer and fixed in 100 μL Fixation buffer (ThermoFisher, #00-8222-49) for 15 min on ice. ..

FACS:

Staining:

Article Title: Phospho-mimic βIII-tubulin rescues microtubule and cardiac defects in Duchenne muscular dystrophy mice.
Article Snippet: After 1 h of attachment onto laminin-coated coverslips (CellTreat 229,172) in 37 ◦C incubator, cardiomyocytes were chilled for 1.5 h in icy water to depolymerize microtubules, then rewarmed at 37 ◦C for the indicated periods (minutes): 0 (directly fix without warm-up), 1, 3, 5, 8, 10, 15. .. Cells were fixed in fixation buffer (4% paraformaldehyde, 60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 1 mM MgCl2, 0.5% Triton X-100, pH 6.9) for 10 min at 37 ◦C, blocked with 1% BSA in PBS for 20 min at room temperature, and stained for α-tubulin (Abcam Ab7291, 1:1000, mouse) for 1 h at room temperature, followed by anti-mouse Alexa 555 (Invitrogen A21424, 1:250) for 1 h at room temperature [92]. ..

Article Title: IL-24 producing regulatory T and B lymphocytes in endometriosis
Article Snippet: Anti-human CD19 FITC (Biolegend, clone: HIB19), CD24 APC-Cy7 (Biolegend, clone: ML5), CD27 Pacific Blue (Biolegend, clone: M-T271), and CD38 Alexa Fluor 700 (Biolegend, clone: HB-7) antibodies were added for Bregs phenotyping, whereas anti-human CD4 Pacific Blue (Biolegend, clone: OKT4), CD25 APC (Invitrogen, clone: CD25-3G10), and CD127 Alexa Fluor 700 (Biolegend, clone: A019D5) antibodies were used for Tregs phenotyping. .. Samples were then washed with staining buffer (0.5 mM EDTA, 0.002% sodium azide, 1% fetal bovine serum) and fixed with a fixation buffer (eBioscience, USA) for 14 h at 4°C in the dark. .. After two washes with permeabilization buffer (eBioscience, USA), samples were blocked with Human True Stain FcX (Biolegend, USA) for 10 min at 4°C in the dark.